Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis

Figure 12 Quercetin ameliorates IVDD invivo. A: Schematic of the basic process of in vivo experiment (By Figdraw); B: Macroscopic appearances of rat tail intact disc mid-sagittal sections in control group, intervertebral disc degeneration group and quercetin group (Scar bar = 2 mm); C: The X-ray in different groups at 0 wk and 4 wk after puncturing; D: Measurements of intervertebral disc height index (DHI); E: Quantitative analysis of DHI; F-G: Hematoxylin-eosin staining and quantitative analysis of histological score at 4 wk after puncture in different groups (scale bar = 1mm); H: The expression of aggrecan and collagen type II in different groups (scale bar = 400 μm); I-J: Quantitative analysis of aggrecan and collagen type II in different groups. Data are represented as mean ± SD. Significant differences between groups are indicated as ^aP < 0.05, n = 3. HE: Hematoxylin-eosin; NP: Nucleus pulposus; AF: Annulus fibrosus; IDD: Intervertebral disc degeneration; TBHP: Tert-butyl hydroperoxide; Que: Quercetin.