Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis

Figure 8 Downregulation of miR-34a-5p alleviated oxidative stress-induced nucleus pulposus-derived mesenchymal stem cells senescence. A: The RNA levels of miR-34a-5p in nucleus pulposus-derived mesenchymal stem cells (NPMSCs) were transfected with miR-34a-5p inhibitor and treated with tert-butyl hydroperoxide; B: The protein levels of SIRT1, p16, p21, p53, IL-1β, IL-6 and MMP-13 in NPMSCs transfected with miR-34a-5p inhibitor; C-I: Quantitative analysis of protein expression in the different groups. Data are represented as mean ± SD. Significant differences between groups are indicated as ^aP < 0.05, n = 3. TBHP: Tert-butyl hydroperoxide.