Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis

Figure 7 miR-34a-5p represses SIRT1 expression by targeting the 3'-UTR of SIRT1. A: Schematic of the predicted miR-34a-5p binding sites in the 3'-UTR of SIRT1 mRNA from TargetScan online database; B: Luciferase reporter activity of SIRT1 co-transfected with miR-34a-5p nc or miR-34a-5p mimic; C-E: The protein and mRNA expression level of SIRT1 in nucleus pulposus-derived mesenchymal stem cells (NPMSCs) transfected with miR-34a-5p mimic; F-H: The protein and mRNA expression level of SIRT1 in NPMSCs transfected with miR-34a-5p inhibitor; I-K: The protein and mRNA expression level of SIRT1 in NPMSCs transfected with Si-SIRT1. Data are represented as mean ± SD. Significant differences between groups are indicated as ^aP < 0.05, n = 3. TBHP: Tert-butyl hydroperoxide.