Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis

Figure 4 Mitochondrial membrane potential assay and reactive oxygen species assay. A: Results of Mitochondrial membrane potential (MMP) in different groups detected by fluorescence. Red fluorescence represents the mitochondrial aggregate JC-1 and green fluorescence indicates the monomeric JC-1. Scale bar = 100 μm; B: Quantitative analysis of MMP results; C: Results of ROS in different groups detected by fluorescence. Red fluorescence represents high level of reactive oxygen species assay (ROS). Scale bar = 200 μm; D: Quantitative analysis of ROS results. Data are represented as mean ± SD. Significant differences between groups are indicated as ^aP < 0.05, n = 3. TBHP: Tert-butyl hydroperoxide; Que: Quercetin; ROS: Reactive oxygen species assay.