Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis

Figure 3 Senescence-associated β-Galactosidase staining assay and cell cycle assay. A: Senescence-associated β-Galactosidase (SA-β-gal) staining results of nucleus pulposus-derived mesenchymal stem cells (NPMSCs) in different groups. Senescent cells exhibit high expression of SA-β-gal in blue staining; B: Cell cycle results of NPMSCs in different groups. Scar bar = 200 μm. Data are represented as mean ± SD. Significant differences between groups are indicated as ^aP < 0.05, n = 3. TBHP: Tert-butyl hydroperoxide; Que: Quercetin.