Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis

Figure 2 Cell viability assay and cell proliferation assay. A: Cell counting kit-8 (CCK-8) results of nucleus pulposus-derived mesenchymal stem cells (NPMSCs) treated with different concentrations of quercetin for 24 h; B: CCK-8 results of NPMSCs treated with different concentrations of tert-butyl hydroperoxide (TBHP) for 24 h; C: EdU assay results of NPMSCs in different groups. Green fluorescence represents cells in a proliferating state, and blue fluorescence represents cell nucleus (scale bar = 200 μm); D: Quantitative analysis of EdU results. Data are represented as mean ± SD. ^aP < 0.05 compared with control group, n = 3. TBHP: Tert-butyl hydroperoxide; Que: Quercetin.