Wnt signaling pathway inhibitor promotes mesenchymal stem cells differentiation into cardiac progenitor cells in vitro and improves cardiomyopathy in vivo

Figure 3 Cardiac markers gene expression analysis by quantitative real-time polymerase chain reaction. A: Seven days treatment of mesenchymal stem cells (MSCs) with inhibitor Wnt production-4 (IWP-4); B: Fourteen days treatment of MSCs with IWP-4 in comparison with untreated control showing significant increase in the expression of early cardiac markers, GATA-4, Nkx2.5, and late cardiac markers, MHC-β, MLC-2v, Mef-2D, cTnT, cTnC, cTnI, α-actinin, Ca-channel and Na-channel. Quantitative two fold (2^-ΔΔCT) difference of mean is represented by ΔΔCt method. Statistical analysis was performed using an Independent sample t-test. Values are presented as mean ± SEM from three independent biological triplicates; level of significance is P < 0.05 (^aP < 0.05, ^bP < 0.01, and ^cP < 0.001). IWP-4: Inhibitor Wnt production-4; MSC: Mesenchymal stem cell; NS: No significant.