Wnt signaling pathway inhibitor promotes mesenchymal stem cells differentiation into cardiac progenitor cells in vitro and improves cardiomyopathy in vivo

Figure 2 Cytotoxicity analysis of mesenchymal stem cells treated with different concentrations of inhibitor Wnt production-4 by JC-1 assay. A: Mesenchymal stem cells (MSCs) treated with 5 μM concentration of inhibitor Wnt production-4 showed non-significant number of apoptotic cells, while 10 μM concentration or above showed significant cytotoxic effect on MSCs; B: Quantification of apoptotic and non-apoptotic cells presented in bar graphs. For statistical analysis, One-way ANOVA was used followed by the Bonferroni post-hoc test. Values are expressed as means ± SEM; level of significance is P < 0.05 (^aP < 0.05, ^cP < 0.001).