Wnt signaling pathway inhibitor promotes mesenchymal stem cells differentiation into cardiac progenitor cells in vitro and improves cardiomyopathy in vivo

Figure 1 Isolation, propagation, and characterization of human umbilical cord-derived mesenchymal stem cells. A: Explant processing and isolation of human umbilical cord-derived mesenchymal stem cells (MSCs). Phase contrast images showing homogenous fibroblast like morphology and growth; B: Characterization of MSCs by immunocytochemistry representing the positive expression of CD117, CD29, Lin28, CD105, Vimentin, and negative expression of CD45; C: Immunophenotyping of MSCs by flow cytometry showing positive expression of CD105, Vimentin, and CD73, and negative expression of CD45; D: Tri-lineages differentiation of MSCs into osteogenic, adipogenic, and chondrogenic lineages.