Constitutive aryl hydrocarbon receptor facilitates the regenerative potential of mouse bone marrow mesenchymal stromal cells

Figure 4 The cluster of differentiation (CD) 86 and CD206 expressions of RAW 264. 7 cells that directly co-cultured with different mouse bone marrow mesenchymal stromal cells. A: The brightfield images of the coculture system (10 objective lens); B: The typical four-quadrant images of flow cytometry after direct coculture of RAW 264.7 cells and mBMSCs (X-axis: F4/80; Y-axis: CD86); C: The typical four-quadrant images of flow cytometry after direct coculture of RAW 264.7 cells and mBMSCs (X-axis: F4/80; Y-axis: CD206); D: Quantitative analysis of CD86 + (Q1-UR)/F4/80 + (Q1-UR + Q1-LR) and CD206 + (Q2-UR)/F4/80 + (Q2-UR + Q2-LR) ratios in direct coculture system. oe-NC: Overexpression-negative control; oe-AhR: Overexpression-aryl hydrocarbon receptor; sh-NC: Knockdown-negative control; sh-AhR: Knockdown-aryl hydrocarbon receptor; NS: Not significant.