Constitutive aryl hydrocarbon receptor facilitates the regenerative potential of mouse bone marrow mesenchymal stromal cells

Figure 3 The effect of aryl hydrocarbon receptor overexpression or knockdown on the osteogenic differentiation in mouse bone marrow mesenchymal stromal cells. A: Alkaline phosphatase (ALP) staining (upper) at 7^th day and Alizarin red staining staining (lower) at 14^th day of osteogenic induction; B: Relative mRNA expression of ALP, biomineralization associated [tissue-nonspecific alkaline phosphatase (ALPL)] and runt-related transcription factor 2 (RUNX2) of overexpression-negative control and overexpression-aryl hydrocarbon receptor (AhR) mouse bone marrow mesenchymal stromal cells (mBMSCs); C: Relative ALPL and RUNX2 mRNA expression of knockdown-negative control and knockdown-AhR mBMSCs at 7^th day of osteogenic induction; D: Representative images of western blot of ALPL and RUNX2 in mBMSCs of different groups at 7^th day of osteogenic induction. oe-NC: Overexpression-negative control; oe-AhR: Overexpression-aryl hydrocarbon receptor; sh-NC: Knockdown-negative control; sh-AhR: Knockdown-aryl hydrocarbon receptor; ALPL: Tissue-nonspecific alkaline phosphatase; RUNX2: Runt-related transcription factor 2; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.