Interferon-gamma and tumor necrosis factor-alpha synergistically enhance the immunosuppressive capacity of human umbilical-cord-derived mesenchymal stem cells by increasing PD-L1 expression

Figure 1 Interferon-gamma induced the expression of programmed cell death protein 1 ligand 1 in human umbilical-cord-mesenchymal stem cells. A-C: Programmed cell death protein 1 ligand 1 (PD-L1) expression was measured by quantitative real-time PCR (qRT-PCR), flow cytometry, and western blot in human umbilical-cord-mesenchymal stem cells (hUC-MSCs) treated with 0, 10, 20, 40, 60, 80 or 100 ng/mL interferon-gamma (IFN-γ) for 24 h; D-F: PD-L1 expression was measured by qRT-PCR, flow cytometry, and western blot in hUC-MSCs treated with 20 ng/mL IFN-γ for 0, 2, 4, 6, 8, 12, 24 or 48 h. Data were represented as mean ± SEM of n = 3. Iso: Isotype; C: Control; ^aP < 0.01; ^bP < 0.001. IFN-γ: Interferon-gamma; PD-L1: Programmed cell death protein 1 ligand 1.