Wnt signaling pathway inhibitor promotes mesenchymal stem cells differentiation into cardiac progenitor cells in vitro and improves cardiomyopathy in vivo

Table 2 Details of Wnt pathway primers

Genes	Primer sequences (5’-3’)	Annealing temperatures (ºC)	Product size (bp)
GAPDH (F)	5′-CAC CAT GGG GAA GGT GAA GG-3’	58.0	274
GAPDH (R)	5′-AGC ATC GCC CCA CTT GAT TT-3’
Wnt-2 (F)	5’-GTCGGGAATCTGCCTTTGTT-3’	58.0	376
Wnt-2 (R)	5’-GTTTTCCTGAAGTCGGCCAT-3’
DVL (F)	5’CTATGGATCAGGATTTCGGGGT-3’	58.0	121
DVL (R)	5’-ATCTCGGGTTGGGGATTATCTG-3’
GSK 3β (F)	5’-TGTGTTGGCTGAGCTGTTACTA-3’	58.0	200
GSK 3β (R)	5’-TGAAATGTCCTGTTCCTGACGA-3’
β-catenin (F)	5’-TGATATTGGTGCCCAGGGAG-3’	58.0	102
β-catenin (R)	5’-TCCATACCCAAGGCATCCTG-3’
c-myc (F)	5’-CACTAACATCCCACGCTCTGA-3’	58.0	217
c-myc (R)	5’-CGCATCCTTGTCCTGTGAGTA-3’
cyc-D (F)	5’-CAGAGGCGGAGGAGAACAAA-3’	58.0	219
cyc-D (R)	5’-CCGGGTCACACTTGATCACT-3’
c-jun (F)	5’-TGAGCCTACAGATGAACTCTTTCT-3’	58.0	191
c-jun (R)	5’-ACTCAGAGTGCTCCAAATCTCTTA-3’
TCF (F)	5’-CGAGAAGAGCAGGCCAAGTA-3’	58.0	219
TCF (R)	5’-GAGCACTGTCATCGGAAGGA-3’
Axin (F)	5’-GCATGGAGGAGGAAGGTGAG-3’	58.0	165
Axin (R)	5’-CCAGGATGCTCTCAGGGTTC-3’