Wnt signaling pathway inhibitor promotes mesenchymal stem cells differentiation into cardiac progenitor cells in vitro and improves cardiomyopathy in vivo

Table 1 Details of cardiac specific primers

Gene	Primer sequences (5’-3’)	Annealing temperatures (ºC)	Product size (bp)
α-actinin (F)	5’-CTCTTGCTTCTACCACGCTTT-3’	58.0	297
α-actinin (R)	5’-AGCGTGTTGAAGTTGATCTCC-3’
cMHC (F)	5’-GAGGAGCAAGCCAACACCA-3’	58.0	106
cMHC (R)	5’-GCAGCTTGTTGACCTGGGA-3’
Mef2D (F)	5’-GGGGGCTGGAGGAGTTACC-3’	58.0	292
Mef2D (R)	5’-TGGGGGAACGGTGTTGTCA-3’
MLC-2v (F)	5’-CCTGAGGAAACCATTCTCAACG-3’	58.0	98
MLC-2v (R)	5’-GATGTGCACCAGGTTCTTGTAG-3’
cTnC (F)	5’-CTCAACCCCAAATCCCCCGA-3’	58.0	148
cTnC (R)	5’-AGGAAGCGGCCATTGGGTAA-3’
cTnI (F)	5’-GAACATCACGGAGATTGCAGA-3’	58.0	236
cTnI (R)	5’-TCAGTGCATCGATGTTCTTCC-3’
cTnT (F)	5’-TCCAGAAGGCCCAGACAGAG-3’	58.0	89
cTnT (R)	5’-CACCTTCCTCCTCTCAGCCA-3’
Ca- channel (F)	5’-GAGAGCACCCCGGCTTC-3’	58.0	164
Ca-channel (R)	5’-GAAGTCCTGCCCCGCTC-3’
Na-channel (F)	5’-ACTAGGCAATTTGTCGGCTC-3’	58.0	325
Na-channel (R)	5’-GCCGTTCTTGAGCAGGTAAT-3’
GATA4 (F)	5’-CTGCCCTCCGTCTTCTGC-3’	58.0	286
GATA4 (R)	5’-CTCGCAGGTCAAGGAGCC-3’
Nkx-2.5 (F)	5’-CAAGTGTGCGTCTGCCTTTC-3’	58.0	106
Nkx-2.5 (R)	5’-CGCGCACAGCTCTTTCTTT-3’
GAPDH (F)	5′-CAC CAT GGG GAA GGT GAA GG-3’	58.0	274
GAPDH (R)	5′-AGC ATC GCC CCA CTT GAT TT-3’