Generation of a human haploid neural stem cell line for genome-wide genetic screening

Figure 6 Summary graph for this research. Results show that human haploid embryonic stem cells cultured in optimized medium can stably maintain haploidy state and easily generate functional haploid neural stem cells with the help of Y27632 (ROCKi), thus contributing to human lineage-specific genetic screening. At last, we get a gene list relate to Mn2⁺-induced toxicity with the help of this haploid cell line. FACS: Fluorescence activated cell sorting.