Transplantation of human induced pluripotent stem cell derived keratinocytes accelerates deep second-degree burn wound healing

Figure 6 COL7A1 knockdown abolishes the facilitative effect of human induced pluripotent stem cells derived keratinocytes on keratinocyte migration in mouse burn wounds. A: On the 14^th day after operation, frozen sections showed that carboxifluorescein diacetate succinimidyl ester labeled human induced pluripotent stem cells derived keratinocytes (hiPSCs-KCs) migrated to the epithelium of the wound healing site scale bar: 100 µm; B: Transwell assay was employed to determine the migration capability of hiPSCs-KCs transfected with COL7A1 shRNA, scale bar: 100 µm; C: Damage repair assay was applied to test the migration capability of hiPSCs-KCs transfected with COL7A1 shRNA. Data: Mean ± SEM. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. n = 10. PBS: Phosphate buffered saline; hiPSCs: Human induced pluripotent stem cells; KCs: Keratinocytes; CFSE: Carboxifluorescein diacetate succinimidyl ester.