Transplantation of human induced pluripotent stem cell derived keratinocytes accelerates deep second-degree burn wound healing

Figure 3 COL7A1 was significantly under-expressed in deep II burn tissues. A: RT-qPCR analysis of COL7A1 mRNA expression in 26 humans deep second degree burn wound tissues and 26 normal skin tissues; B: Western blotting analysis of COL7A1 protein expression in 3 human deep second degree burn wound tissues and 3 normal skin tissues; C: Representative photomicrographs of hematoxylin-eosin stained burned skin of mice at 24 h post burn, scale bar: 100 µm; D: Immunohistochemical detection of COL7A1 expression levels in burn wounds and surrounding normal skin of mice, scale bar: 100 µm; E: Immunofluorescence detection of COL7A1 expression levels in keratinocytes, hiPSCs and fibroblasts, scale bar: 50 µm. Data: Mean ± SEM. ^bP < 0.01 vs Normal group.