Commitment of human mesenchymal stromal cells to skeletal lineages is independent of their morphogenetic capacity

Figure 4 Cell death and fibroblast growth factor 8 expression pattern in mesenchymal stromal cells and chicken recombinant limbs at 24 h postimplantation. Lysotracker staining (Ltk) to evaluate cell death in recombinant limbs (RLs) from the placenta (PL-RLs; n = 6), umbilical cord blood (UCB-RLs; n = 6), and chicken (CK-RLs; n = 4) is shown. In situ hybridization of fibroblast growth factor 8 (Fgf8) in PL-RLs showed disrupted pattern of this gene in 2/4 RLs; Fgf8 expression in UCB-RLs was detected in 3/6 evaluated samples, while 5/5 CK-RLs continued to express Fgf-8 at 24 h postimplantation. UCB: Umbilical cord blood; PL: Placenta; RL: Recombinant limb; CK: Chicken; Ltk: Lysotracker staining; Fgf8: Fibroblast growth factor 8.