Injectable hydrogel made from antler mesenchyme matrix for regenerative wound healing via creating a fetal-like niche

Table 1 In vitro experiment design

Test purposes	Cell type	Culture system					Culture time	Test type		Required reagents
Screen the optimum concentration of HARM on cell culture	HUVEC	HARM (0 μg/mL)	HARM (10 μg/mL)	HARM (20 μg/mL)	HARM (30 μg/mL)	HARM (40 μg/mL)	7 d	CCK-8 assay		CCK-8 (Beyotime, Shanghai, China)
	NIH 3T3
	ADSC
	BMSC
Compare the performance of HARM to HUPB on cell proliferation	ADSC	HARM (20 μg/mL)	P-CTRL (HUPB, 20 μg/mL)		N-CTRL (PBS)		1, 4, 7, 10 d	CCK-8 assay	Nuclear staining	CCK-8 (Beyotime, Shanghai, China)
	BMSC

HUVEC: Human umbilical vein endothelial cells; NIH 3T3: Mouse embryonic fibroblasts; ADSC: Adipose mesenchymal stem cells; BMSC: Bone marrow mesenchymal stem cells; HARM: Hydrogels from antler reserve mesenchyme; HUPB: Hydrogel from porcine urinary bladder matrix; P-CTRL: Positive control; N-CTRL: Negative control.