Human retinal secretome: A cross-link between mesenchymal and retinal cells

Table 2 Secretome and extracellular vesicle features

Type	Dimension	Origin	Collection	Content
Medium-size EVs (microvesicles)	200-1000 nm	Plasma membrane shedding	Filtration, ultracentrifugation, chromatography, precipitation, immunoaffinity	Similar to exosomes + cytosolic/plasma membrane/post-translational modified proteins
Small-size EVs (exosomes)	Up to 200 nm	Multivesicular bodies pathway	Filtration, ultracentrifugation, chromatography, precipitation, immunoaffinity	Receptors, transcription factors, enzymes, proteins, lipids, nucleic acids (DNA, mRNA and miRNA)
Soluble factors	Up to 5 nm	Protein synthesis	Protein extraction methods	Proteins, growth factors, chemokines, cytokines, enzymes
Secretome	From protein size to 1000 nm	Cell secretion/shedding	Cell culture media (concentration is possible)	The combination of the other components

EV: Extracellular vesicle; miRNA: MicroRNA.