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©The Author(s) 2023.
World J Stem Cells. Jun 26, 2023; 15(6): 589-606
Published online Jun 26, 2023. doi: 10.4252/wjsc.v15.i6.589
Published online Jun 26, 2023. doi: 10.4252/wjsc.v15.i6.589
Figure 4 Cd271 knockout mesenchymal stem cells showed weaker proliferation and osteogenic differentiation potential.
A: Gene Ontology enrichment analysis of differentially expressed genes between Cd271 knockout and wild-type mesenchymal stem cells (MSCs); B: Growth curves of Cd271 knockout and wild-type MSCs; C: Mineralization assay of Cd271 knockout and wild-type MSCs. Alkaline phosphatase (ALP) and Alizarin red staining of Cd271 knockout MSCs was significantly lighter. ALP activity and calcium quantification were significantly lower in Cd271 knockout compared with wild-type MSCs; D: Protein–protein interaction network interaction analysis of wildtype vs Cd271 knockout differentially expressed genes; E: Quantitative real-time polymerase chain reaction assay of some differentially expressed genes in Cd271 knockout and wild-type MSCs. Data are presented as mean values ± SD (n = 3 biologically independent experiments. Two-sided unpaired t-test, aP < 0.05, bP < 0.01, cP < 0.001) BP: Biological Process; CC: Cell component; MF: Molecular function; ARS: Alizarin red S; ALP: Alkaline phosphatase; KO: Knockout; WT: Wild-type.
- Citation: Zhang YY, Li F, Zeng XK, Zou YH, Zhu BB, Ye JJ, Zhang YX, Jin Q, Nie X. Single cell RNA sequencing reveals mesenchymal heterogeneity and critical functions of Cd271 in tooth development. World J Stem Cells 2023; 15(6): 589-606
- URL: https://www.wjgnet.com/1948-0210/full/v15/i6/589.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v15.i6.589