Exosomes from circ-Astn1-modified adipose-derived mesenchymal stem cells enhance wound healing through miR-138-5p/SIRT1/FOXO1 axis regulation

Figure 4 Exosomes from circular RNA astrotactin 1-modified adipose-derived mesenchymal stem cells have greater therapeutic effect in promoting wound healing in a diabetic mouse model. A: Representative images of full-thickness skin defects after treatment with adipose-derived mesenchymal stem cell (ADSC) exosomes or circular RNA astrotactin 1-modified ADSC exosomes for 0, 1, and 2 wk after wounding; B and C: Microvascular formation evaluated by immunofluorescence staining with cluster of differentiation 31. ^bP < 0.001 vs control. ^dP < 0.001 vs exosomes; D and E: We assayed apoptosis level via terminal deoxynucleotidyl transferase dUTP nick end labeling staining. ^bP < 0.001 vs control. ^dP < 0.001 vs exosomes; F: Hematoxylin and eosin staining shows wound changes; G-I: Quantitative polymerase chain reaction and western blot analysis showing mi-138-5p (G), SIRT1 (H), and forkhead box O1 (I) expression. ^bP < 0.001 vs control. ^dP < 0.001 vs exosomes.