Exosomes from circ-Astn1-modified adipose-derived mesenchymal stem cells enhance wound healing through miR-138-5p/SIRT1/FOXO1 axis regulation

Figure 3 The circular RNA astrotactin 1-mediated mi-138-5p/SIRT1/forkhead box O1 signaling pathway plays an important protective role in endothelial precursor cells under high glucose conditions by promoting angiogenesis. A and B: Luciferase expression levels in HEK293 cells transfected with cloned circular RNA astrotactin 1 (circ-Astn1) wild-type (WT) or mutant (MUT) vector and miR-138-5p mimics. Data are denoted by the mean ± SD. ^bP < 0.001; C: Quantitative polymerase chain reaction (qPCR) detection suggested that miR-138-5p expression was reduced after transfection with circ-Astn1-overexpressing vector in endothelial precursor cells (EPCs). Data are denoted as the mean ± SD. ^bP < 0.001 vs NC; D-G: Representative photomicrographs of tube-like structures of EPCs under high glucose (HG) conditions after transfection with negative control or circ-Astn1-overexpressing vector. ^bP < 0.001 vs HG. ^dP < 0.001 vs circ-Astn1; H and I: Luciferase expression level in HEK293 cells transfected with cloned SIRT1 WT- or MUT-3' UTR vector and miR-138-5p mimics. Data are denoted by the mean ± SD. ^bP < 0.001; J and K: qPCR and western blot analysis indicated that SIRT1 and forkhead box O1 expression were reduced after transfection with miR-138-5p overexpression vector in EPCs. Data are expressed as the mean ± SD. ^bP < 0.001 vs NC. ^dP < 0.001 vs miR-138-5p mimics; L-O: Representative photomicrographs of EPC tube-like structures under HG conditions after transfection with miR-138-5p mimics combined with or without SIRT1 overexpression vector. Data are denoted by the mean ± SD. ^bP < 0.001 vs NC. ^dP < 0.001 vs miR-138-5p mimics.