Exosomes from circ-Astn1-modified adipose-derived mesenchymal stem cells enhance wound healing through miR-138-5p/SIRT1/FOXO1 axis regulation

Figure 2 Exosomes derived from circular RNA astrotactin 1-modified adipose-derived mesenchymal stem cells function importantly in endothelial precursor cell function restoration by decreasing apoptosis under high glucose conditions. A: Heat map regarding all differentially expressed circular RNAs (circRNAs) between adipose-derived mesenchymal stem cells (ADSCs) exosomes and fibroblast exosomes; B: Quantitative polymerase chain reaction giving mmu_circ_0000101 (circular RNA astrotactin 1), mmu_circ_0008040, mmu_circ_0008061, and mmu_circ_0008099 expression in endothelial precursor cells (EPCs) with or without high glucose (HG) treatment. Data are denoted by the mean ± SD; ^bP < 0.001 vs normal; C: The genomic loci of circ-Astn1; D and E: We pretreated EPCs with ADSC exosomes before treatment with exosomes for 1 d under HG conditions. Our team assayed EPC apoptosis via flow cytometry after annexin V-FITC staining. ^bP < 0.001 vs normal. ^dP < 0.001 vs HG; F: EPC proliferation under different treatments, determined by Cell Counting Kit-8 assay. ^bP < 0.001 vs normal. ^dP < 0.001 vs HG; G-J: Representative photomicrographs of tube-like structures. Scale bar: 50 μm. Technician-counted tube branch points (H), relative tube length (I) and the total number of branches were calculated. ^bP < 0.001 vs normal. ^dP < 0.001 vs HG. PI: Propidium iodide.