Repetitive administration of cultured human CD34+ cells improve adenine-induced kidney injury in mice

Figure 2 CD34+ cell and endothelial progenitor cell colony-forming units expansion by quality and quantity culture method. A: Number of total CD34+ cells. The plot graph demonstrates CD34+ positivity per 1 × 10⁴ fresh UCB-CD34+ sample and post-culture CD34+ cell number per 1 × 10⁴ initially seeded cells; B: Expansion rate of CD34+ cells. The expansion rate of CD34+ cells from fresh CD34+ cells (day 0) to cultured CD34+ cells (day 7); C: Endothelial progenitor cells (EPC) colony-forming unit. The number of TEPC-CFUs, primitive EPC-CFUs, and definitive EPC-CFUs of post- quality and quantity culture (QQc) CD34+ cells. Gray column: Significantly increased compared with that of pre-QQc CD34+ cells; black column: Statistical significances in Figure 2A-C were determined using Mann-Whitney U test. UCB: Umbilical cord blood; EPC: Endothelial progenitor cell; CFU: Colony-forming unit; QQc: Quality and quantity culture; TEPC: Total endothelial progenitor cell; PEPC: Primitive endothelial progenitor cell; DEPC: Definite endothelial progenitor cell.