Bone marrow mesenchymal stem cell-derived exosomal microRNAs target PI3K/Akt signaling pathway to promote the activation of fibroblasts

Figure 6 Phosphatase and tensin homolog is verified as the targets of miR-144-3p and miR-23b-3p. A: Human and mouse sequence of phosphatase and tensin homolog (PTEN) for miR-144-3p binding site; B: Human and mouse sequence of PTEN for miR-23b-3p binding site; C: The relative luciferase activity was tested after co-transfection of WT/MUT pmirGLO-PTEN-3’UTR and miR-144-3p mimics or their control groups in NIH3T3 cells; D: The relative luciferase activity was tested after co-transfection of WT/MUT pmirGLO-PTEN-3’UTR and miR-23b-3p mimics or their control groups in NIH3T3 cells; E: The mRNA levels of PTEN in NIH3T3 cells transfected with miR-144-3p mimics or miR-144-3p inhibitors; F: The mRNA levels of PTEN in NIH3T3 cells transfected with miR-23b-3p mimics or miR-23b-3p inhibitors; G: Western blot showing the PTEN expression levels after transfected with miR-144-3p mimics and miR-23b-3p mimics. n = 3 per groups. Data are shown as mean ± SD. ^aP < 0.05, ^bP < 0.01.