Mammalian Ste20-like kinase 1 inhibition as a cellular mediator of anoikis in mouse bone marrow mesenchymal stem cells

Figure 5 The properties and biological safety of mouse bone marrow mesenchymal stem cells/sh-Mammalian Ste20-like kinase 1. A: The levels of interleukin-4 (IL-4), IL-10, IL-17A and IL-6 were measured using a Cytometric Bead Array Cytokine Kit in detached mouse bone marrow mesenchymal stem cells (mBMSCs); B: Representative images of alkaline phosphatase (ALP) staining of mBMSCs after culture in osteogenesis induction medium for 21 d; C: Quantitative real-time polymerase chain reaction analysis of the relative mRNA expression levels of runt-related transcription factor 2 (Runx2) and ALP in mBMSCs cultured in adipogenesis induction medium for 21 d; D: Nude mice were subcutaneously implanted with mBMSCs (n = 3), mBMSCs/NC-Mst1 (n = 3), and mBMSCs/sh-Mst1 (n = 4). Representative images of the heart, liver, spleen, lung and kidney in each group 60 d postinjection; E. Paraffin-embedded subcutaneous sections derived from cell-injection sites were stained with haematoxylin and eosin. Data are the mean ± SD of three technical replicates. mBMSCs: Mouse bone marrow mesenchymal stem cells; Mst1: Mammalian Ste20-like kinase 1; IL: Interleukin; Runx2: Runt-related transcription factor 2; ALP: Alkaline phosphatase. ^cP < 0.01; ^bP < 0.05; ^aP > 0.05.