ADSC-Exos outperform BMSC-Exos in alleviating hydrostatic pressure-induced injury to retinal ganglion cells by upregulating nerve growth factors

Figure 1 Adipose-derived stem cell and bone marrow-derived stem cell-exosomes, and retinal ganglion cells were isolated and identified. A: The immunofluorescence staining of stem cell markers SOX2 (Scale bar: 20 μm); B: The morphology of adipose-derived stem cell-exosomes (ADSC-Exos) and bone marrow-derived stem cell (BMSC)-Exos was observed by electron microscopy (scale bar: 200 nm); C: The ADSC-Exos and BMSC-Exos marker molecules (CD9, CD63, and CD81) were detected by western blotting; D: The light microscope images of retinal ganglion cells (RGCs) (magnification: 40 × and 400 ×); E: The isolated RGCs was examined by Thy1.1 immunofluorescence (scale bar: 50 μm). ADSC: Adipose-derived stem cell; BMSC: Bone marrow-derived stem cell; Exo: Exosome.