Dissecting molecular mechanisms underlying ferroptosis in human umbilical cord mesenchymal stem cells: Role of cystathionine γ-lyase/hydrogen sulfide pathway

Figure 1 Characteristics of human umbilical cord mesenchymal stem cells expressing firefly luciferase gene. A: Luciferase activity of human umbilical cord mesenchymal stem cells expressing firefly luciferase gene (HUCMSCs-LUC). Representative luciferase imaging is shown using in vivo imaging system; B: Morphologies of HUCMSCs-LUC. HUCMSCs-LUC differentiated into adipocytes (stained with oil red O for lipid droplets) and osteocytes (stained with alizaline red for mineral deposition); C: Surface marker expression of HUCMSCs-LUC in flow cytometry analysis. HUCMSCS: Human umbilical cord mesenchymal stem cells; HUCMSCs-LUC: Human umbilical cord mesenchymal stem cells expressing firefly luciferase gene; PE: Phycoerythrin; FITC: Fluorescein isothiocyanate.