Hypoxia and inflammatory factor preconditioning enhances the immunosuppressive properties of human umbilical cord mesenchymal stem cells

doi:10.4252/wjsc.v15.i11.999

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Nov 26, 2023 (publication date) through Jul 30, 2024

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Stem Cells. Nov 26, 2023; 15(11): 999-1016
Published online Nov 26, 2023. doi: 10.4252/wjsc.v15.i11.999

Table 1 Primer used to amplify the human transcripts during real-time quantitative polymerase chain reaction

Gene	Sequence, 5’ to 3’	Application
Catalae (human)	Upper: TTCTGTTGAAGATGCGGCGAGAC	qRT-PCR
Catalae (human)	Lower: GGGGTACTTTCCTGTGGCAATGG	qRT-PCR
STC-1 (human)	Upper: CCATGAGGCGGAGCAGAATGAC	qRT-PCR
STC-1 (human)	Lower: GCCGACCTGTAGAGCACTGTTG	qRT-PCR
HMOX1 (human)	Upper: TGCCAGTGCCACCAAGTTCAAG	qRT-PCR
HMOX1 (human)	Lower: TGTTGAGCAGGAACGCAGTCTTG	qRT-PCR
Bax (human)	Upper: ACCAAGAAGCTGAGCGAGTGTC	qRT-PCR
Bax (human)	Lower: TGTCCACGGCGGCAATCATC	qRT-PCR
Bcl2 (human)	Upper: TGAGTTCGGTGGGGTCATGT	qRT-PCR
Bcl2 (human)	Lower: TTCCACAAAGGCATCCCAGC	qRT-PCR
SIRT1 (human)	Upper: CCTGGACAATTCCAGCCATCT	qRT-PCR
SIRT1 (human)	Lower: TGCAAAGGAACCATGACACTGA	qRT-PCR
P53 (human)	Upper: GCCCATCCTCACCATCATCACAC	qRT-PCR
P53 (human)	Lower: GCACAAACACGCACCTCAAAGC	qRT-PCR
P16 (human)	Upper: CCGTGGACCTGGCTGAGGAG	qRT-PCR
P16 (human)	Lower: CGGGGATGTCTGAGGGACCTTC	qRT-PCR
P21 (human)	Upper: CTTGTACCCTTGTGCCTCGC	qRT-PCR
P21 (human)	Lower: AGAAGATCAGCCGGCGTTTG	qRT-PCR
β-actin (human)	Upper: AGTGTGACGTGGACATCCGCA	qRT-PCR
β-actin (human)	Lower: ATCCACATCTGCTGGAAGGTGGAC	qRT-PCR
PGE2 (human)	Upper: ATTCTCCTGGCTATCATGAC	qRT-PCR
PGE2 (human)	Lower: GAACAGGAGGCCTAAGGATG	qRT-PCR
IDO (human)	Upper: CTCTGCCAAATCCACAGGAAA	qRT-PCR
IDO (human)	Lower: ATGACCTTTGCCCCACACAT	qRT-PCR
KYN (human)	Upper: CAAGCGAAGGGTTGTTATGTTGGC	qRT-PCR
KYN (human)	Lower: GGAACACCAGCAGGCAAAATCAAC	qRT-PCR
COX2 (human)	Upper: AATCTGGCTGCGGGAACACAAC	qRT-PCR
COX2 (human)	Lower: TGTCTGGAACAACTGCTCATCACC	qRT-PCR
IL-1ra (human)	Upper: GTGCCTGTCCTGTGTCAAGTCTG	qRT-PCR
IL-1ra (human)	Lower: GCCACTGTCTGAGCGGATGAAG	qRT-PCR
IL-10 (human)	Upper: GCCAAGCCTTGTCTGAGATGATCC	qRT-PCR
IL-10 (human)	Lower: GCCTTGATGTCTGGGTCTTGGTTC	qRT-PCR
TGF-β1 (human)	Upper: AGCAACAATTCCTGGCGATACCTC	qRT-PCR
TGF-β1 (human)	Lower: TCAACCACTGCCGCACAACTC	qRT-PCR
HLA-G5 (human)	Upper: AGAGGAGACACGGAACACCAAGG	qRT-PCR
HLA-G5 (human)	Lower: CAGGTCGCAGCCAATCATCCAC	qRT-PCR
TSG-6 (human)	Upper: AGAGAAGCACGGTCTGGCAAATAC	qRT-PCR
TSG-6 (human)	Lower: GCCATCCATCCAGCAGCACAG	qRT-PCR
PD-L1 (human)	Upper: TGACCTACTGGCATTTGCTGAACG	qRT-PCR
PD-L1 (human)	Lower: CACTGCTTGTCCAGATGACTTCGG	qRT-PCR

Bax: B-cell lymphoma 2-associated protein; Bcl2: B-cell lymphoma 2; COX2: Cyclooxygenaese-2; HLA-G5: Human leukocyte antigen-G5; HOMX1: Hemeoxygenase-1; IDO: Idoleamine-2,3-dioxygenase; IL-1ra: Interleukin-1 receptor antagonist; IL-10: Interleukin-10; KYN: Kynurenine; PD-L1: Ligands for programmed cell death 1; PGE2: Prostaglandin E2; qRT-PCR: Quantitative real-time polymerase chain reaction; SIRT1: Silencing information regulator 2-related enzyme 1; STC1: Stanniocalcin-1; TGF-β1: Transforming growth factor-beta 1; TSG-6: Tumor necrosis factor-α-induced protein-6.

Citation: Li H, Ji XQ, Zhang SM, Bi RH. Hypoxia and inflammatory factor preconditioning enhances the immunosuppressive properties of human umbilical cord mesenchymal stem cells. World J Stem Cells 2023; 15(11): 999-1016
URL: https://www.wjgnet.com/1948-0210/full/v15/i11/999.htm
DOI: https://dx.doi.org/10.4252/wjsc.v15.i11.999