MicroRNA-584-5p/RUNX family transcription factor 2 axis mediates hypoxia-induced osteogenic differentiation of periosteal stem cells

Figure 3 Prediction of the miRNA-584-5p target. A: Potential binding sites of miRNA-584-5p and the 3’-untranslated region of RUNX family transcription factor 2 (RUNX2); B: Dual luciferase reporter gene assay; C: Quantitative polymerase chain reaction determination of RUNX2 expression; D: Western blot determination of RUNX2 protein expression. One-way ANOVA and t tests were used to demonstrate differences in RUNX2 responses between the miR584-5p mimic and negative control groups. ^aP < 0.05; ^bP < 0.01. RUNX2: RUNX family transcription factor 2; NC: Negative control; wt: Wild type; mut: Mutant; miRNA: MicroRNA.