Long non-coding RNA SNHG16 promotes human placenta-derived mesenchymal stem cell proliferation capacity through the PI3K/AKT pathway under hypoxia

Figure 6 SNHG16 overexpression resulted in activation of the PI3K/AKT pathway and a significant enhancement in the proliferative rate of human placenta-derived mesenchymal stem cells. A: Quantitative reverse transcription polymerase chain reaction analysis of relative SNHG16 expression after transfection of lentivirus overexpressing SNHG16 (SNHG16-OE) and the corresponding empty vector in human placenta-derived mesenchymal stem cells; B: Cell proliferation after SNHG16 overexpression was evaluated by cell counting kit-8 assay; C: Cell cycle distribution after SNHG16 overexpression was evaluated by flow cytometry; D: The expression levels of CDK2, CDK4, CDK6, cyclin E1, cyclin D1, and phosphorylated AKT. Data are presented as the means ± SD obtained from three separate experiments. ^bP < 0.01.