Long non-coding RNA SNHG16 promotes human placenta-derived mesenchymal stem cell proliferation capacity through the PI3K/AKT pathway under hypoxia

Figure 1 Hypoxia facilitated human placenta-derived mesenchymal stem cell growth and proliferation. A: Western blot analysis of hypoxia-inducible factor 1α expression in human placenta-derived mesenchymal stem cells (hP-MSCs) under hypoxic culture for 24, 48, or 72 h; B: Morphology of the cultured hP-MSCs under hypoxia (scale bars, 100 μm); C: Proliferation curves of hP-MSCs were established based on cumulative cell numbers at different incubation times (0, 1, 2, 3, and 4 d) under normoxia or hypoxia; D: Colony size and colony number of hP-MSCs under normoxic or hypoxic culture (n = 6); E: The protein expression of c-MYC and proliferating cell nuclear antigen in hP-MSCs under hypoxic culture for 24, 48, or 72 h. Data are presented as means ± SD. ^aP < 0.05. NS: No significance; PCNA: Proliferating cell nuclear antigen; HIF-1α: Hypoxia-inducible factor 1α.