Overexpression of GATA binding protein 4 and myocyte enhancer factor 2C induces differentiation of mesenchymal stem cells into cardiac-like cells

Figure 2 Human umbilical cord mesenchymal stem cell characterization by immunocytochemical analysis, flow cytometry, and lineage differentiation assays. A: Immunocytochemsitry of human umbilical cord mesenchymal stem cell (hUC-MSC) showing positive expression of CD44, CD90, CD105, and vimentin, and negative expression of CD45, a hematopoietic marker. Images were captured under a fluorescence microscope (scale bar: 50 μm); B: Flow cytometry of hUC-MSCs showing positive expression of CD44, CD73, and CD90. Data were analyzed using BD FACS Diva software; C: Adipogenic and osteogenic lineage differentiation of hUC-MSCs. Images were captured under a phase contrast microscope (scale bar: 100 μm).