Overexpression of GATA binding protein 4 and myocyte enhancer factor 2C induces differentiation of mesenchymal stem cells into cardiac-like cells

Table 1 Primer sequence and annealing temperature of cardiac genes

Gene	Primer sequence (5’-3’)	Annealing temperature (°C)
Beta-actin	Forward: 5’-TGGGCATGGGTCAGAAGGATTC-3’	60
	Reverse: 5’-AGGTGTGGTGCCAGATTTTCTC-3’
Myocyte enhancer factor 2C	Forward: 5’-CGAGATGCCAGTCTCCATCC-3’	60
	Reverse: 5’-CAGAGAAGGGTGAGCCAGTG-3’
NKX2.5	Forward: 5’-AGTGTGCGTCTGCCTTTCC-3’	60
	Reverse: 5’-CACAGCTCTTTCTTTTCGGCTC-3’
MHC	Forward: 5’-GACAGGTGCAGCAAAA CAGG-3’Reverse: 5’-AAGGGTATCCTGCAACTGCC-3’	60
Connexin-43	Forward: 5’-CTTCATGCTGGTGGTGTCC-3’	60
	Reverse: 5’-ACCACTGGTCGCATGGTAAG-3’
GATA-4	Forward: 5’-CTGCCCTCCGTCTTCTGC-3’	60
	Reverse: 5’-CTCGCAGGTCAAGGAGCC-3’

NKX2.5: NK2 homeobox 5; MEF2C: Myocyte enhancer factor 2C; MHC: Myosin heavy chain; GATA-4: GATA binding protein 4.