Combination of mesenchymal stem cells and three-dimensional collagen scaffold preserves ventricular remodeling in rat myocardial infarction model

Figure 9 Analysis of angiogenesis. A: Immunohistochemical analysis of rat hearts by α-smooth muscle actin showing blood vessels in the left ventricle in sham control and myocardial infarction model; scaffold only group, and untreated and zebularine treated mesenchymal stem cells (MSC)-seeded scaffold groups also show angiogenesis in the scaffold region as well; B: Bar graphs showing the quantification of blood vessels and statistical analysis. Zebularine treated MSC-seeded scaffold transplanted group showed more significant increase in the number of blood vessels. Data is presented as mean ± standard error of mean with significance level ^aP < 0.05 (where ^aP < 0.05, ^bP < 0.01, ^cP < 0.001). SMA: Smooth muscle actin; MI: Myocardial infarction; MSC: Mesenchymal stem cells.