Combination of mesenchymal stem cells and three-dimensional collagen scaffold preserves ventricular remodeling in rat myocardial infarction model

Figure 5 Protein expression analysis of cardiac markers. A: GATA4; B: Cardiac troponin T (cTnT); C: Nkx2.5. Fluorescence images showing the expression of cardiac specific proteins GATA4, cTnT, and Nkx2.5 in frozen sections of zebularine treated mesenchymal stem cells (MSC)-seeded collagen scaffold. Corresponding bar graphs representing the percentage of positive cells and their respective fluorescence intensities in treated and untreated MSC-seeded collagen scaffolds. Data is presented as mean ± standard error of mean with significance level ^aP < 0.05 (where ^aP < 0.05, ^bP < 0.01, ^cP < 0.001). cTnT: Cardiac troponin T; DAPI: 4',6-diamidino-2-phenylindole.