Combination of mesenchymal stem cells and three-dimensional collagen scaffold preserves ventricular remodeling in rat myocardial infarction model

Figure 2 Isolation and characterization of mesenchymal stem cells. A: Passage 0 and Passage 1 mesenchymal stem cells (MSCs) in culture showing plastic adherence and fibroblast like appearance, and homogeneous population; B: Immunocytochemical analysis showing positive expression of CD29, CD44, CD90, CD117, vimentin, and negative expression of CD45 which is a hematopoietic marker; C: Flow cytometric analysis showing > 95% positive expression of MSC specific surface markers CD29, CD44, CD90; D: Trilineage differentiation analysis of MSCs by Alizarin Red S, Oil Red O and Alcian Blue staining showing the ability of MSCs to differentiate into osteogenic, adipogenic and chondrogenic lineages, respectively. DAPI: 4',6-diamidino-2-phenylindole.