Changes of cell membrane fluidity for mesenchymal stem cell spheroids on biomaterial surfaces

Figure 5 Membrane fluidity of mesenchymal stem cells, 3T3, and A549 individually cultured on chitosan-hyaluronan substrates. The single cell suspension was subjected to the analysis for respective ratios of the cells labeled with single and double fluorescent dyes. A: Illustration of experimental procedures; B: Mesenchymal stem cells, A549, or 3T3 cells labeled with fluorescent dyes were cultured on chitosan-hyaluronan substrates for 3 d, and then the membrane fluidity of cells was revealed by the percentages of the double-labeled cell population. ^bP < 0.01, among the indicated groups. Scale bar: 100 μm. MSC: Mesenchymal stem cells.