Sinomenine promotes differentiation of induced pluripotent stem cells into immature dendritic cells with high induction of immune tolerance

Figure 4 Sinomenine-induced pluripotent stem cells–immature dendritic cells suppress T cell proliferation and enhanced regulatory T cell proliferation. A: Purity of CD3⁺ T cells after sorting; B: Mixed lymphocyte reaction at different proportions of immature dendritic cells: CD3⁺ T cells. Compared with induced pluripotent stem cells-dendritic cells (iPSCs-DCs) group, ^aP < 0.05; C: Purity of CD4⁺ T cells after sorting; D: Mixed culture of iPSCs-DCs or sinomenine (SN)-iPSCs-DCs and CD4⁺ T cells at 1:40. Compared with iPSCs-DCs group, ^aP < 0.05; E: Effects of iPSCs-DCs or SN-iPSCs-DCs on regulatory T (Treg) cell proliferation. Compared with iPSCs-DCs group, ^aP < 0.05; F: Cytokine levels in supernatants from cocultured DCs and Treg cells. Compared with iPSCs-DCs group, ^aP < 0.05. SN: Sinomenine; iPSCs: Induced pluripotent stem cells; DCs: Dendritic cells; IL: Interleukin; IFN: Interferon; TGF-: Transforming growth factor-.