How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models

Table 3 Bone marrow cotransplantation model

Ref.	HSC donor	MSC donor	Host	Conditioning	Source	Dose (Gy)	MSC immunogenicity	Groups
Lee et al[9]	Mice (C57BL/6)	Human	Mice (BALB/c H-2d)	Chemotherapy	Bu/Cy	25 mg/kg/4 d	Xenogeneic	Control; HSC; HSC + T-MSC; HSC + MMP3kdT-MSC
						100 mg/kg/2 d
Huang et al[10]	Human	Human	Mice (NOD/SCID)	TBI	60-Co	2.7	Xenogeneic	Control; HSC; HSC + UCB-MSC (IV/IB)
Yin et al[8]	Human	Human	Mice (NOD/SCID; NOG)	TBI	NR	2.5	Xenogeneic	HSC; HSC + GFP-MSC; HSC + EGF-MSC; HSC + FGF2-MSC; HSC + PDGFB-MSC (IV/IB)
Choi et al[11]	Mice (C57BL/6)	Human	Mice (BALB/c)	Chemotherapy	Bu/Cy	20 mg/kg/4 d	Xenogeneic	Control; HSC; HSC + T-MSC
						100 mg/kg/2 d
Trento et al[12]	Mice (C57BL/6 CD45.1)	Mice (C57BL/6 CD45.2 orNos2-/-)	Mice (C57BL/6 CD45.2)	TBI	NR	8	Allogeneic	HSC; HSC + MSC; HSC + Nos2−/−MSC
Abbuehl et al[13]	Mice (FVB Actin-GFP)	Mice (FVB Insulin-GFP)	Mice (FVB Insulin-GFP)	TBI	NR	2 × 4.5	Allogeneic	HSC; HSC + MSC
Kim et al[14]	Mice (C57BL/6J-Pep3b-Ly5.1 Pep3b)	Mice (C57BL/6J-Ly 5.2 BL6)	Mice (C57BL/6J-Ly 5.2 BL6)	TBI	NR	9	Xenogeneic	HSC; HSC + MSC (NSS/SS) direct or priming
Futrega et al[15]	Human	Human	Mice (NSG)	TBI	137-Cs	2.5	Xenogeneic	HSC (IV/IB); HSC + MSC; HSC + MSC-spheroids (IB)
van der Garde et al[16]	Human	Human	Mice (NOD/SCID)	TBI	NR	3.5	Xenogeneic	HSC; HSC + MSC; Ex/TPO-HSC; Ex/TPO-HSC + MSC
Fernández-García et al[17]	Mice (P3D2F1 H2b/d CD45.1)	Mice (B6D2F1,H2b/d, CD45.2)	Mice (B6D2F CD45.2)	TBI	NR	5-7	Allogeneic	HSC; HSC + AT-MSC
Chen et al[18]	Mice (C57BL/6)	Mice (C57BL/6)	Mice (BALB/c H-2d)	TBI	60-Co	7.5	Allogeneic	HSC; HSC + EGFP-MSC; HSC + CXCR4-MSC
Chen et al[19]	Human	Human	Mice (NOD/SCID/IL2rγnull)	TBI	60-Co	3.5	Xenogeneic	Control; HSC; HSC + MSC; HSC + SDF-1-MSC; HSC + HOXB4-MSC; HSC + SDF1-HOXB4-MSC
Wu et al[20]	Human	Human	Mice (NOD/SCID)	TBI	NR	3.5	Xenogeneic	HSC; HSC + UCB-MSC
Lim et al[21]	Human	Human	Mice (NOD/SCID)	TBI	Cs	2.5	Xenogeneic	HSC; HSC + hPTH; HSC + UCB-MSC + hPTH
Lee et al[22]	Human	Human	Mice (NOD/SCID)	TBI	NR	3	Xenogeneic	HSC; HSC + BM-MSC; HSC + AT-MSC; HSC + UCB-MSC
Kornblit et al[23]	Dog (beagle)	Dog (beagle)	Dog (Beagle)	TBI	60-Co	9.2	Allogeneic	Control; HSC; HSC + MSC (unrelated or DLA-id MSC)
Fortin et al[24]	Mice (B6.SJL-PtrcaPep3b/BoyJ CD45.1)	Mice	Mice (C57BL/6J)	TBI	60-Co	8	Allogeneic	HSC; HSC + MSC; HSC + solG-CSFR-MSC
Carrancio et al[25]	Human	Human	Mice (NOD/SCID)	TBI	Cs	3	Xenogeneic	HSC (IV/IB); HSC IV + MSC (IV/IB)

Bu: Busulfan; Cy: Cyclophosphamide; TBI: Total body irradiation; Cs: Cesium; Co: Cobalt; HSC: Hematopoietic stem cells; Ex/TPO-HSC: HSC expanded with thrombopoietin; MSC: Mesenchymal stem cells; T-MSC: Tonsil mesenchymal stem cells derived; UCB-MSC: Umbilical cord blood mesenchymal stem cells derived; AT-MSC: Adipose tissue mesenchymal stem cells derived; BM-MSC: Bone marrow mesenchymal stem cells derived; MMP3kdT-MSC: Mesenchymal stem cells with matrix metallopeptidase 3-knockdown; GFP-MSC: Mesenchymal stem cells expressing green fluorescent protein; EGF-MSC: Mesenchymal stem cells expressing epidermic growth factor; FGF2-MSC: Mesenchymal stem cells expressing fibroblast growth factor 2; PDGFB-MSC: Mesenchymal stem cells expressing platelet-derived growth factor subunit B; Nos2^-/-MSC: Mesenchymal stem cells deficient in type 2 nitric oxide; EGFP-MSC: Mesenchymal stem cells expressing enhanced green fluorescent protein; CXCR4-MSC: Mesenchymal stem cells expressing C-X-C chemokine receptor type 4; SDF-1-MSC: Mesenchymal stem cells expressing stromal cell-derived factor 1; HOXB4-MSC: Mesenchymal stem cells expressing Homeobox B4; solG-CSFR-MSC: Mesenchymal stem cells expressing soluble granulocyte colony-stimulating factor decoy receptor; hPTH: Human parathyroid hormone; DLA: Dog leukocyte antigen; DLA-id: Dog leukocyte antigen identical; NSS: Non-stimulatory serum; SS: Stimulatory serum.