Combination of mesenchymal stem cells and three-dimensional collagen scaffold preserves ventricular remodeling in rat myocardial infarction model

doi:10.4252/wjsc.v14.i8.633

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Aug 26, 2022 (publication date) through Nov 28, 2024

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World Journal of Stem Cells

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1948-0210

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World J Stem Cells. Aug 26, 2022; 14(8): 633-657
Published online Aug 26, 2022. doi: 10.4252/wjsc.v14.i8.633

Table 1 Details of cardiac specific gene primers

No.	Gene primers	Primer sequences (5׳-3׳)	Product size
1	GAPDH (F)	GGAAAGCTGTGGCGTGATGG	414
1	GAPDH (R)	GTAGGCCATGAGGTCCACCA	414
2	GATA4 (F)	TCTCACTATGGGCACAGCAG	245
2	GATA4 (R)	CCGAGCAGGAATTTGAAGAG	245
3	cTnT (F)	TTCGACCTGCAGGAAAAGTT	206
3	cTnT (R)	GTGCCTGGCAAGACCTAGAG	206
4	Nkx2.5 (F)	ACCGCCCCTACATTTTATCC	230
4	Nkx2.5 (R)	GACAGGTACCGCTGTTGCTT	230
5	Connexin43 (F)	GCTCCACTCTCGCCTATGTC	234
5	Connexin43 (R)	GAGTTCATGTCCAGCAGCAA	234
6	cMHC (F)	GCAGGTGGATGATCTGGAGG	116
6	cMHC (R)	CCATGATGCTCTCCTGGGTC	116
7	Tbx20 (F)	TGATTCTGGCCCTAGGAAAGTG	100
7	Tbx20 (R)	TGAGCCTGGGTTCTCCATAAAG	100
8	ANF (F)	CCTGCGAAGATCAAGCTGC	110
8	ANF (R)	GCTCGAGCAGATTTGGCTG	110
9	cTnI (F)	GACCTGCGTGGCAAGTTTA	139
9	cTnI (R)	CCTCCTTCTTCACCTGCTTG	139
10	Mef2c (F)	GAGATACCCACAACACACGC	117
10	Mef2c (R)	GAGTGGAATTCGTTCCGGTG	117

F: Forward; R: Reverse; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; cTnT: Cardiac troponin T; cMHC: Cardiac myosin heavy chain; Tbx20: T-box transcription factor 20; ANF: Atrial natriuretic factor; cTnI: Cardiac troponin I; Mef2C: Myocyte-specific enhancer-binding factor 2C.

Citation: Qazi REM, Khan I, Haneef K, Malick TS, Naeem N, Ahmad W, Salim A, Mohsin S. Combination of mesenchymal stem cells and three-dimensional collagen scaffold preserves ventricular remodeling in rat myocardial infarction model. World J Stem Cells 2022; 14(8): 633-657
URL: https://www.wjgnet.com/1948-0210/full/v14/i8/633.htm
DOI: https://dx.doi.org/10.4252/wjsc.v14.i8.633