Soluble factors secreted by human Wharton’s jelly mesenchymal stromal/stem cells exhibit therapeutic radioprotection: A mechanistic study with integrating network biology

Figure 3 Effect of Wharton's jelly-derived mesenchymal stromal/stem cells on homeostasis-driven proliferation of lymphocytes in lymphopenic mice. Wharton's jelly-derived mesenchymal stromal/stem cells (WJ-MSCs) were mixed with carboxyfluorescein diacetate succinimidyl ester (CFSE) labeled lymphocytes were injected into 6 Gy exposed mice. In another group, only CFSE labeled lymphocytes were injected into irradiated mice. A: Dot plot shows gating strategy to monitor the proliferation of lymphocytes after acquiring the samples on flow cytometer; B: Bar graph showing % of CFSE positive lymphocytes with and without WJ-MSCs; C: Flow cytometric histograms showing lymphocyte proliferation; D: Bar graph showing percent daughter cells; E: Bar graph showing spleen index (n = 4 mice per group). ^aP < 0.05; ^bP < 0.005 as compared to radiation alone.