Optimization of adipose tissue-derived mesenchymal stromal cells transplantation for bone marrow repopulation following irradiation

Figure 7 Effect of co-culture on CD34⁺ cell fate. CD34⁺ cells were incubated in the absence (control group) or presence of human adipose tissue-derived stem cells (hADSCs) conditioned medium (CM group) or directly on a hADSC layer (contact group). A: Total cell number; B: Percentage of CD34⁺ cells; C: The number of CD34⁺ cells were determined after 7 d of incubation; D: Colony-forming unit assays were performed using MethoCult™ by plating an equal number of CD34⁺ cells. The number of colonies was counted after 14 d of incubation; E-P: Differentiation assays of bone marrow CD34⁺ cells in liquid cultures, in the absence (control group) or presence of hADSC CM group or directly on a hADSC layer (contact group). The percentage of each surface marker was analyzed using flow cytometry (upper panel) and the marker-positive cell numbers were calculated (lower panel). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, and ^dP < 0.0001 vs controls. CFU: Colony-forming unit; CM: Conditioned media; CFU-E: Colony-forming unit erythroid; BFU-E: Burst-forming unit erythroid.