Extracellular vesicles from hypoxia-preconditioned mesenchymal stem cells alleviates myocardial injury by targeting thioredoxin-interacting protein-mediated hypoxia-inducible factor-1α pathway

Figure 2 Identification of extracellular vesicles. A: Transmission electron microscopy characterization of extracellular vesicles (EVs); B: Size distribution by intensity was detected using the NanoSight Instrument; C: EV biomarkers cluster of differentiation 63 (CD63), CD81, and tumor susceptibility gene 101 were identified by western blotting of adipose-derived mesenchymal stem cell (ADSC)-derived EVs and ADSCs; D: EV tracer assay was used to identify exosomes phagocytosed by cardiomyocytes. ADSC: Adipose-derived mesenchymal stem cell; EV: Extracellular vesicles.