Extracellular vesicles from hypoxia-preconditioned mesenchymal stem cells alleviates myocardial injury by targeting thioredoxin-interacting protein-mediated hypoxia-inducible factor-1α pathway

Figure 1 Identification of adipose-derived mesenchymal stem cells and neonatal mouse cardiomyocytes. A: Flow chart of extracellular vesicles and neonatal mouse cardiomyocyte (CM) isolation; B: Characterization of adipose-derived mesenchymal stem cells (ADSCs) was performed by flow cytometry analyses of cluster of differentiation 34 (CD34), CD105, and CD106 (negative controls) and CD29, CD45, and CD90 (positive cell surface markers); C: Identification of neonatal mouse CMs, which were specifically stained with α-actinin. CM: Cardiomyocyte; ADSC: Adipose-derived mesenchymal stem cells; CD: Cluster of differentiation.