Transcription regulators differentiate mesenchymal stem cells into chondroprogenitors, and their in vivo implantation regenerated the intervertebral disc degeneration

Figure 7 Gene expression analysis of transfected human umbilical cord mesenchymal stem cells via quantitative polymerase chain reaction. A: Bar graphs with significant transcriptional expression of transforming growth factor beta-1 gene (TGFβ1), BMP, Sox-9, and Six-1 at 48 h post-transfection; B, C: Significant expression of TGFβ1, BMP, Sox-9, and aggrecan observed at 21 d post-transfection shows long term sustainability in the expression pattern. However, the expression was time-dependent, Six-1 was significantly downregulated in both normal and chondro-induction media at day 21. ^aP < 0.05 vs control; ^bP < 0.01 vs control; ^cP < 0.001 vs control.