Transcription regulators differentiate mesenchymal stem cells into chondroprogenitors, and their in vivo implantation regenerated the intervertebral disc degeneration

Figure 2 Transfection efficiency of human umbilical cord mesenchymal stem cells. A: Positive expression of Sox-9, and Six-1 proteins was observed after 48 h of electroporation, as analyzed by immunocytochemical staining; B: Quantification of fluorescent intensities showed that Sox-9 transfected cells expressed Sox-9 protein, Six-1 transfected mesenchymal stem cells (MSCs) expressed Six-1 protein, and Sox-9+Six-1 transfected MSCs expressed Sox-9, and Six-1 proteins. ^aP < 0.05 vs control; ^bP < 0.01 vs control.