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©The Author(s) 2022.
World J Stem Cells. Dec 26, 2022; 14(12): 822-838
Published online Dec 26, 2022. doi: 10.4252/wjsc.v14.i12.822
Published online Dec 26, 2022. doi: 10.4252/wjsc.v14.i12.822
Figure 7 SPOC domain-containing protein 1 promotes the proliferation of spermatogonial stem cells via adenylate kinase 4.
A and B: Western blotting shows the level of adenylate kinase 4 (AK4) after re-expression in SPOC domain-containing protein 1 (SPOCD1) knockdown spermatogonial stem cells (SSCs); C: Cell Counting Kit-8 was used to detect cell proliferation after AK4 was overexpressed in SPOCD1-knockdown SSCs; D and E: Cellular DNA synthesis was detected by 5-ethynyl-2’-deoxyuridine (EdU) analysis; F and G: Western blotting shows the protein levels of promyelocytic leukemia zinc finger (PLZF) and proliferating cell nuclear antigen (PCNA) after transfection with AK4 expression plasmid in SPOCD1 inhibited SSC; H and I: Cell apoptosis analysis after re-expression of AK4 using flow cytometry. Scale bar in D: 20 μm. aP < 0.05.
- Citation: Zhou D, Zhu F, Huang ZH, Zhang H, Fan LQ, Fan JY. SPOC domain-containing protein 1 regulates the proliferation and apoptosis of human spermatogonial stem cells through adenylate kinase 4. World J Stem Cells 2022; 14(12): 822-838
- URL: https://www.wjgnet.com/1948-0210/full/v14/i12/822.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v14.i12.822