SPOC domain-containing protein 1 regulates the proliferation and apoptosis of human spermatogonial stem cells through adenylate kinase 4

Figure 5 Effects of SPOC domain-containing protein 1 inhibition on apoptosis of human spermatogonial stem cells. A and B: Flow cytometry and APC Annexin V analysis show proportions of early and late apoptotic cells in human spermatogonial stem cells (SSCs) transfected with negative control (NC)-small interfering RNA (siRNA) and SPOC domain-containing protein 1-siRNA 2; C and D: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis shows proportions of TUNEL-positive cells in human SSCs transfected with NC-siRNA and forkhead box P4 (FOXP4)-siRNA 2. Scale bars in C: 20 μm. ^aP < 0.05.